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Figure 6 | Respiratory Research

Figure 6

From: JNK suppresses pulmonary fibroblast elastogenesis during alveolar development

Figure 6

JNK regulates expression of many elastin-associated genes. (A-C) The lungs of PND14 JNK-deficient mice contained more emilin-1, fibrillin-1, and fibulin-5 mRNAs than those of wild type mice. (D) mRNA of lysyl oxidase was modestly increased in lungs of JNK-deficient mice. (E) Tropoelastin mRNA was increased in the lungs of JNK-deficient mice. All lungs were from three PND14 mice from at least two different litters. (F-I) Emilin-1, fibrillin-1, fibulin-5, and lysyl oxidase mRNAs were elevated in PND14 JNK-deficient lung fibroblasts compared to wild type. Emilin-1 and fibulin-5 mRNAs were not detected in PND14 wild-type lung fibroblasts, so for statistical comparisons, a Ct value of 40 cycles was assumed. (J) A simplified schematic depicts elastin fibril assembly. Tropoelastin (Eln) forms a multimeric complex with binding proteins Fibulin-5 (Fbln5), and the cross-linking enzyme lysyl oxidase (LOX). This multimeric complex then binds to microfibrils composed of fibrillin-1 (Fbn1). Fibrillin-1 assembly is facilitated by emilin which binds to cells via an integrin-binding domain. *p < 0.05, **p < 0.01, ***p < 0.001 by one-way ANOVA. All analyses were performed in triplicate.

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