Figure 1

Clonal expansion of early passage human ASM cells. Early passage human ASM cells were single sorted by fluorescence-activated cell sorting and subcultured in 20% or 10% FCS containing DMEM medium at 37°C, 5% CO₂ atmosphere. All images were taken from the same well and used ×40 objective (A) Single human ASM cell as seen in culture on day 2 (see black arrow). (B) Single human ASM cell proliferating to give rise to a number of cells (day 5). (C) Further expansion at day 18.