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Table 4 miRNA expression changes in healthy ASM cells after treatment with FCS or Dex + FCS

From: Role of non-coding RNAs in maintaining primary airway smooth muscle cells

miRNA

Baseline expression (ΔCT)

FCS

Dex + FCS

Microarray (FC)

qRT-PCR (FC)

Microarray (FC)

qRT-PCR (FC)

hsa-miR-134

6.1 (<.001)

-

-

−1.5 (<.001)

−1.32 (<.05)

hsa-miR-150*

6.2 (<.001)

-

-

−1.5 (<.001)

−1.25 (<.05)

hsa-miR-371-5p

6.3 (<.001)

−1.6 (<.001)

−0.97 (<.05)

−1.7 (<.001)

−1.73 (<.05)

hsa-miR-718

5.4 (<.001)

−1.5 (<.001)

−1.26 (<.05)

−1.6 (<.001)

−1.44 (<.05)

hsa-miR-762

7.6 (<.001)

−1.7 (<.001)

-

−1.9 (<.001)

-

hsa-miR-940

6.7 (<.001)

−1.5 (<.001)

-

−1.6 (<.001)

-

hsa-miR-1181

7.0 (<.001)

−1.7 (<.001)

−0.89 (<.05)

−1.8 (<.001)

−0.89 (<.05)

hsa-miR-1207-5p

7.7 (<.001)

−1.8 (<.001)

−1.73 (<.05)

−2.0 (<.001)

−1.13 (<.05)

hsa-miR-1915

9.0 (<.001)

−1.8 (<.001)

−0.58 (<.05)

−1.9 (<.001)

−0.66 (<.05)

hsa-miR-3663-3p

8.0 (<.001)

−1.8 (<.001)

−1.10 (<.05)

−2.0 (<.001)

−1.36 (<.05)

hsa-miR-3665

9.7 (<.001)

−2.1 (<.001)

-

−2.3 (<.001)

-

hsa-miR-4271

6.1 (<.001)

-

-

−1.5 (<.001)

-

hsa-miR-4281

9.6 (<.001)

−1.6 (<.001)

-

−1.5 (<.001)

-

  1. This table lists those miRNAs that were differentially expressed in healthy ASM cells following stimulation with FCS (2.5%) or treatment with dexamethasone (10–7 M) before stimulation with FCS (2.5%). Baseline expression levels are expressed as Δ cycle threshold (ΔCT) and have been normalized against RNU44. Microarray data are denoted as the fold change against baseline control and is the mean of 9 individual patients. These values are for those genes that demonstrated an FDR of less than 0.05; associated P values are shown in parentheses. FC, Fold change.