Figure 7

Pulmonary delivery of pVR1020-HA pDNA induces antibody response in rats. Systemic and mucosal (a) IgG and (b) IgA antibody responses detected in the sera of female Sprague-Dawley rats (n=8 per group) 3 weeks following 300 μg pVR1020-HA pDNA vaccination (primary immunization) encoding an influenza A virus surface antigen, human hemagglutinin (HA) via lung instillation. Bars represent the mean ± SD and individual rats are indicated as symbols. No significant (NS) difference between the pre and post-SAW nebulized vaccine instillation was found (p=0.163 for IgG and p=0.486 for IgA, respectively), and a significant difference between these and the naïve rat was found (p<0.001). Note that the antibody response detected in the naïve rat group represents the detection limit of the assay.