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Figure 2 | Respiratory Research

Figure 2

From: Macrophage activation state determines the response to rhinovirus infection in a mouse model of allergic asthma

Figure 2

Airway inflammation and airway hyperresponsiveness in OVA-treated wild-type and IL-4R KO mice. Eight-week old wild-type or IL-4R KO mice were treated with PBS or OVA and inoculated with sham or RV. Bronchoalveolar lavage was performed 24 hour post-inoculation. After counting the total number of cells, cytospins were performed and stained with hematoxylin and eosin, and differential counts determined from 200 cells. The identity of neutrophils and eosinophils was confirmed by immunofluorescence staining for neutrophil elastase and major basic protein (not shown). (A) RV infection increases the total number of BAL cells per lung in OVA-sensitized and -challenged wild-type and IL-4R KO mice. (B) RV infection increases the number of airway neutrophils per lung in OVA-treated wild-type and IL-4R KO mice. The neutrophil response was significantly higher in IL-4R KO mice. (C) RV infection increases the number of airway eosinophils per lung in OVA-treated wild-type mice. The eosinophil response was significantly attenuated in IL-4R KO mice. (Mean ± SEM, n = 3, *different from medium, p < 0.05, one-way ANOVA; †different from wild-type, p < 0.05, one-way ANOVA.) (D & E) Airway cholinergic responsiveness was assessed by measuring changes in total respiratory system resistance in response to increasing doses of nebulized methacholine. Data from wild type (D) and IL-4R KO mice (E) are shown. (Mean ± SEM, n = 4-6 in each group, *different from sham, P < 0.05, two-way ANOVA; †different from PBS, P < 0.05, two-way ANOVA).

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