Figure 4

At E13.5, Cldn6 immunofluorescence was co-localized with FoxA2 (A-C), Gata-6 (D-F), Sox2 (G-I), and Sox9 (J-L). Merged images are shown (A, D, G and J) that include Cldn6 (B, E, H, and K), and FoxA2 (C), Gata-6 (F), Sox2 (I), or Sox9 (L). DAPI staining was performed for cellular perspective. No immunoreactivity was observed in lung sections incubated without primary antibodies (not shown) and all images are at 40X original magnification. Scale bars represent 20 μm.