Skip to main content

Table 1 Studies utilizing microarray technology to analyze IPF

From: Application of microarray technology in pulmonary diseases

Investigator

Microarray type

Species/Sample size

Summary/Key findings

Normalization procedure

 

Number of genes

Type of tissue

 

Replications per data point

Zuo et al.22

Oligonucleotide 8.400 genes

5 patients with IPF Lung tissue specimens

Gene expression analysis reveals matrilysin as a key regulator of PF in mice and humans.

Gene expression levels normalized by a scaling factor multiplied to the average of differences of probe pairs (matched-mismatched) / 2 replicates

Kaminski et al.23

Oligonucleotide 6.000 genes

30 mice Lung tissue specimens

Global analysis of gene expression in PF reveals distinct programs regulating lung inflammation and fibrosis.

Mean hybridization intensities of all probe sets on each array were scaled to an arbitrary, fixed level/4 replicates

Katsuma et al. 24

cDNA 4.224 genes

22 mice Lung tissue samples

Molecular monitoring of bleomycin-induced pulmonary fibrosis by cDNA microarray-based gene expression profiling.

Quantified signal intensities were converted by logarithms of base two 4 replicates

Chambers et al. 25

Oligonucleotide 6.000 genes

Human lung fibroblasts

Global expression profiling of fibroblast responses to transforming growth factor-beta1 reveals the induction of ID1.

Gene expression levels normalized by a scaling factor multiplied to the average of differences of probe pairs (matched-mismatched)/ 2 replicates

Liu et al.26

cDNA 10.000 genes

12 rats Lung tissue specimens

FIZZ1 stimulation of myofibroblast differentiation.

Average median ratios Cy3/Cy5 values normalized to 1.0/ 4 replicates

  1. Abbreviations: ID: Inhibitor of Differentiation, IPF: Idiopathic Pulmonary Fibrosis, PF: Pulmonary Fibrosis