From: Application of microarray technology in pulmonary diseases
Investigator | Microarray type | Species/Sample size | Summary/Key findings | Normalization procedure |
---|---|---|---|---|
 | Number of genes | Type of tissue |  | Replications per data point |
Lee et al.34 | Oligonucleotide 6.500 genes | Human airway cells | IL-13 induces dramatically different transcriptional programs in three human airway cell types. | Gene expression levels normalized by a scaling factor multiplied to the average of differences of probe pairs (matched-mismatched)/ 1 replicate |
Temple et al.35 | Oligonucleotide 6800 genes | Human eosinophils | Microarray analysis of eosinophils reveals a number of candidate survival and apoptosis genes. | Geometric mean of the scaling (standard experiment) factor served as normalization factor/ 2 replicates |
Hakonarson et al.36 | Oligonucleotide 5.000 genes | Rabbit and human ASM | Association between IL-1beta/TNF-alpha-induced glucocorticoid-sensitive changes in multiple gene expression and altered responsiveness in ASM. | Gene expression levels normalized by a scaling factor multiplied to the average of differences of probe pairs (matched-mismatched) / 2 replicates |
Laprise et al.37 | Oligonucleotide 12.000 probe sets | 8 subjects Lung tissue samples | Functional classes of bronchial mucosa genes that are differentially expressed in asthma. | Mean hybridization intensities of all probe sets on each array were scaled to a fixed level/ 2 replicates |
Nakajima et al.39 | Oligonucleotide 12.000 genes | Human MCs and eosinophils | Gene expression screening of human mast cells and eosinophils using high-density oligonucleotide probe arrays: abundant expression of MBP in MCs | Mean hybridization intensities of all probe sets on each array were scaled to an arbitrary, fixed level / 1 replicate |