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Figure 5 | Respiratory Research

Figure 5

From: Effects of cigarette smoke on degranulation and NO production by mast cells and epithelial cells

Figure 5

Effects of CSS on iNOS mRNA from mouse AEC by real-time PCR. Quantitative real-time RT-PCR was performed to determine mouse iNOS mRNA levels. Quantification of mRNA was performed by determining the threshold cycle and standard curves were constructed using the values obtained from serially diluted positive control mouse iNOS plasmid, and determining the values for experimental samples from these curves. A progressive fall in copy number was seen in AEC iNOS expression with 1% CSS, over 24 h. Data points represent 3 replicates with S.E.M.

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