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Table 1 PCR primers as used in Methods.

From: Effects of cigarette smoke on degranulation and NO production by mast cells and epithelial cells

NOS isoform

Primer sequence

Rat iNOS [26]

sense:5'-GGACCACCTCTATCAGGAA-3', antisense 5'-CCTCATGATAACGTTTCTGGC-3';

Rat eNOS [27]

sense: 5'-TACCAGCCGGGGGACCAC-3', antisense: 5'-CGAGCTGAC-AGAGTAGTA-3'.

Human iNOS [28]

sense: 5'-GAGCTTCTACCT-CAAGCTATC-3', antisense: 5'-CCTGATGTTGCCATTGTTGGT-3';

Human eNOS

sense:5'-GCACAGGAA-ATGTTCACC TAC-3', antisense: 5'-CACGATGGTGAC-TTTGGCTAG-3'.

Mouse iNOS (real time PCR) probe

sense: 5'-CAGCTGGGCTGTACAAACCTT-3', antisense: 5'-CATTGGAAGTGAAGCGTTTCG-3', 5'-6FAM (fluorescent reporter dye, 6-carboxyfluorescein)-CGGGCAGCCTGTGAGACCTTTGA-TAMRA (quenching agent, 6-carboxytetramethylrhodamine, Applied Biosystems, CA, USA).