Skip to main content
Figure 2 | Respiratory Research

Figure 2

From: TGF-β1 and serum both stimulate contraction but differentially affect apoptosis in 3D collagen gels

Figure 2

TUNEL staining in HFL-1 cells. Fibroblast-populated collagen gels were released into 60 mm tissue culture dishes with or without FCS or TGF-β1. On day 5, collagen gels were digested, cells isolated, cytocentrifuge preparation made, and stained by TUNEL. A: positive control (DNAse-1 treated), B: negative control (without terminal transferase), C: FCS free, D: FCS 1%, E: TGF-β1(100 pM). Red: PI stained normal cells. Green: TUNEL positive cells. Data presented are from one representative experiment. Similar results were obtained in three experiments performed on separate occasions.

Back to article page