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Table 2 Association rate constant for inhibition of HNE by α1-AT and Z α1-AT with SP-A. Data are means ± SD of experiments performed in triplicate with 3 different batches of human SP-A and1batch of rabbit SP-A.

From: SP-A binds alpha1-antitrypsin in vitro and reduces the association rate constant for neutrophil elastase

α1-AT/SPA ratio

α1-AT

Z α1-AT

 

Reaction condition

Kass, (M-1sec-1)

decrease in K ass, n-fold

Reaction condition

Kass, (M-1sec-1)

decrease in K ass, n-fold

 

α1-AT nM

SP-A nM

  

Z α1-AT nM

SP-A nM

  
 

7.5

0

3.40 ± 0.0079 × 107

0

7.5

0

9.80 ± 0.0032 × 106

0

50

7.5

0.15

1.84 ± 0.0577 × 107

1.8

7.5

0.15

5.20 ± 0.0314 × 106

1.9

5

7.5

1.5

1.70 ± 0.0623 × 107

2

7.5

1.5

4.70 ± 0.0268 × 106

2.1

1

7.5

7.5

5.20 ± 0.0483 × 106

6.5

7.5

7.5

4.30 ± 0.0240 × 106

2.3

0.5

7.5

15

4.30 ± 0.0513 × 106

7.9

7.5

15

3.80 ± 0.0221 × 106

2.6