Figure 5

Western blot analysis of Grx1 in lung homogenates. Representative Western blot analysis of Grx1 expression in the specimens (n = 32) of healthy non-smokers and smokers without COPD and in different stages of COPD (for patient characteristics see Table 2). The total amount of protein was 40 μg and the primary antibody dilution for human Grx1 was 1:2500 in all cases. The means of the intensities measured are shown as columns with error bars representing SEM. * Significantly decreased when compared to smokers (p = 0.045). ** Significantly decreased when compared to smokers (p = 0.022).