Figure 6

Grx1 expression in induced sputum supernatants and plasma samples. Representative Western blot analysis of Grx1 expression in the plasma (A) and induced sputum pellets (B) and supernatants (C) of healthy non-smokers (= NS), smokers (= S) and COPD patients (= C). The means of the intensities in the sputum supernatants were measured from total of 15 non-smokers, 11 smokers, 17 stable COPD (stage 0–I) and 7 acute exacerbations (for patient characteristics see Table 3) and are shown as columns (D). The primary antibody dilution for human Grx1 was 1:2500 in all cases. Error bars represent SEM. * Significantly increased when compared to non-smokers (p = 0.013).