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Figure 4 | Respiratory Research

Figure 4

From: Lung development in laminin γ2 deficiency: abnormal tracheal hemidesmosomes with normal branching morphogenesis and epithelial differentiation

Figure 4

In vitro branching morphogenesis. Lung buds were removed from E12.5 littermate control and Lamc2-/- embryos and grown in culture for 72 hours. (A) Images of littermate control (a-d) and Lamc2-/- (e-h) lung buds were acquired at time of explant and after 24, 48, and 72 hours in culture. (B) Terminal peripheral airway buds were quantified and averaged (+/- SD) for each time point. No statistically significant differences were observed in littermate control and Lamc2-/- lungs. Analysis was performed with lung buds from 2 separate litters with at least 3 buds each for Lamc2-/- and littermate control. Original magnification 40×.

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