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Figure 2 | Respiratory Research

Figure 2

From: Airway smooth muscle relaxation results from a reduction in the frequency of Ca2+ oscillations induced by a cAMP-mediated inhibition of the IP3 receptor

Figure 2

Relaxation of contracted airways by ISO. (A) A series of phase-contrast images of the same airway (scale bar = 100 μm) at times indicated by arrows in B (middle trace) showing (1) the initial state, (2) the contracted state induced by MCh (100 nM) and (3) the relaxed state induced by ISO (10 μM). (B) The changes in the area of an airway in response to ISO (10 μM) following contraction with MCh. The airway was contracted with decreasing concentrations of MCh (1μM to 10 nM) for 3 min and exposed to the same concentration of ISO (10 μM) for another 3 min. The slices were washed with sHBSS 15 minutes between each concentration of MCh. (C) Summary of the ISO-induced (10 μM) relaxation of airways contracted with different concentrations of MCh (n = 4 slices from 3 mice). The magnitude of the airway relaxation induced by ISO increased as the concentration of MCh was decreased (*, p < 0.05, comparison between two different MCh concentrations).

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Respiratory Research

ISSN: 1465-993X

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