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Figure 8 | Respiratory Research

Figure 8

From: Airway smooth muscle relaxation results from a reduction in the frequency of Ca2+ oscillations induced by a cAMP-mediated inhibition of the IP3 receptor

Figure 8

Effect of flash photolysis of caged-Ca2+on SMCs treated with MCh and FSK. (A) A fluorescence image of part of an airway obtained by confocal microscopy. L, lumen; ECs, epithelial cells; SMC, smooth muscle cell. The dashed white oval indicates the position and size of the zone of UV illumination (10 s, OD 0.6). Dotted line indicates the interface between ECs and SMCs. Scale bar = 40 μm. The intracellular Ca2+ signaling represented by(B) a line-scan plot, constructed by sequentially aligning the pixels along the length of the SMC (white line indicated in A) and from each recorded fluorescence image and (C) a selected ROI within the SMC and (D) the frequency of Ca2+ oscillations of SMCs in response to flash photolysis of caged-Ca2+ during stimulation with MCh (200 nM) and FSK (10 μM). Flash photolysis induced a temporary rise in [Ca2+]i but did not increase the slow frequency of the Ca2+ oscillations. Representative traces of 6 different slices from 4 mice.

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