Figure 1
Salmeterol and salbutamol inhibit TNFα production by mouse alveolar macrophages in vitro. MH-S cells were incubated with 5 × 105 HKNTHi in the presence or absence of salmeterol or salbutamol. A, Effect of salmeterol and salbutamol (both 10-7 M) on the kinetics of NTHi-induced TNFα release. B, Salmeterol and salbutamol inhibited NTHi-induced TNFα release (6 h incubation) in a dose dependent fashion, which can be reversed by propranolol (10-5 M). Data are mean ± SEM of experiments performed in triplo. Please note that error bars fall within the symbols at multiple time points and concentrations. Markers of significance (described in the Results section) were omitted for reasons of clarity (figure 1B).