Figure 5

Effect of IL-6 on the expression of TLR-2 (panel A) and TLR-4 (panel B) by human monocytes from never smoker. Purified monocytes were incubated in the presence or absence of IL-6 (10 ng/ml) and 3 h later TLRs expression was analyzed by flow cytometry. Shaded area represents TLRs staining of monocytes incubated without IL-6 whereas un-shaded area represents TLRs staining of monocytes incubated with IL-6. mfi values of cells incubated with isotype matched antibodies were 4.3 ± 1.6 whereas mfi values of IL-6-treated cells incubated with isotype matched antibodies were 5.4 ± 1.2 (p > 0.05). Inset shows results of three different never smokers tested in duplicate for each condition studied. The results were analyzed by paired two-tailed t test. Symbol: * significant difference (p < 0.05) versus non-treated monocytes.