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Figure 2 | Respiratory Research

Figure 2

From: Role of acetylcholine and polyspecific cation transporters in serotonin-induced bronchoconstriction in the mouse

Figure 2

Effect of epithelium removal on constriction of peripheral bronchi in PCLS of M2/3R+/+ mice. (A) Reduction of luminal area of intact (control, blue) and epithelium-denuded (denuded, red) peripheral bronchi in response to muscarine (Mus, 10-6 M) and 5-HT (10-6 M). The numbers in parentheses refer to the numbers of bronchi/number of lungs from which they were taken. Panel (B) illustrates the magnitude of the response to 5-HT (10-6 M) compared to that to muscarine (10-6 M) which was set as 100 %. Control bronchi react slightly stronger to 5-HT than to muscarine, whereas the 5-HT response is significantly smaller that the muscarine response after epithelium removal, particularly at 1 min (1') after agonist application. The box plots shows percentiles 0, 25, 50 (median), 75, and 100; individual data points beyond 3× S.D. are indicated by * or °. ***p < 0.001, **p < 0.01, *p < 0.05 (comparison of corresponding time points by Mann-Whitney U-test). (C) Microscopic appearance of control and epithelium-denuded bronchi. In the left panel, arrowheads indicate thickness of the epithelial layer in a control bronchus. In the right panel, the arrowhead points to a small remnant of epithelium after mechanical denudation of the epithelium.

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