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Figure 3 | Respiratory Research

Figure 3

From: Toll-like receptor-4 mediates cigarette smoke-induced cytokine production by human macrophages

Figure 3

IL-8 production by human macrophages is TLR4 mediated. a) Cells were incubated overnight with anti-TLR4 or isotype control prior to CS medium or LPS or PMA/ionomycin exposure. IL-8 production was quantified using ELISA. The result is a representative of 3–5 experiments conducted with different human monocyte-derived macrophage preparations in which the mean fold increase in IL-8 production at concentration of CS = 0.03 was 1.8 ± 0.31 (n = 5) and the mean percentage of reduction in IL-8 production in the presence of the anti-TLR4 antibody was 66.7 ± 8.6 (n = 5). b) Cells were incubated with anti-TLR2 or anti-TLR4 prior to CS medium exposure and Cytometric Beads Array assay was performed. The result is a representative of 3 experiments conducted with different human monocyte-derived macrophage preparations that had similar results.

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