Figure 7

CpG increased IL-1β-induced IL-8 mRNA stability. A. Cells were treated with IL-1β in the absence or presence of CpG for 2 h at which time media was changed and actinomycin D was added to the cells to induce transcriptional arrest. Both groups of cells were harvested for total RNA extraction at 15 min intervals, as indicated. The top gel is a representative Northern blot and the bottom gel shows the 18s rRNA levels depicted by ethidium bromide staining of the transferred membrane. B. Decay rate of Northern blot shown in A. Data points are plotted as the percent IL-8 mRNA remaining relative to time zero. Half-life calculations are depicted as the average (± SEM, n = 4 separate experiments) time at which there was 50% IL-8 mRNA remaining under the respective treatments.