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Figure 2 | Respiratory Research

Figure 2

From: Susceptibility to ozone-induced airway inflammation is associated with decreased levels of surfactant protein D

Figure 2

Kinetics of O 3 -induced SP-D and inflammatory changes in C57BL/6 mice. Groups of C57BL/6 mice were exposed to 3 ppm O3 or room air and studied 0, 2, 6, 12, 24, 48, and 72 hrs later. (A): SP-D and SP-A recovered from the BAL were quantified by an in-house ELISA. Results are expressed as % change from naïve controls. SP-D levels gradually increased until the 72 hrs time point (p = 0.0022, ANOVA and post test for linear trend) whereas SP-A levels did not change significantly. (B): The number of neutrophilic granulocytes was assessed by counting total number of BAL cells in the BAL fluid and performing differential cell counting on Kwick™Diff cytospin preparations. Results are expressed as absolute cell number/ml of BAL fluid. Neutrophilic inflammation peaked around 12 hrs post exposure (p < 0.0001) and largely resolved by 72 hrs after O3 exposure. (C-D) IL-6 and IL-10 levels were assessed as part of a Luminex®100™ assay and showed significant increases 2–6 hrs after O3 exposure (p < 0.0001) and 6–24 hrs after O3 exposure (p = 0.0007), respectively. (A-D): Mean ± SEM of n = 6 in each groups.

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