Figure 6

Simvastatin influences cell cycle progression in human lung fibroblasts by inducing G1 arrest. LL97a lung fibroblasts at 60% confluency were serum deprived for 48 hours; cells were then harvested following treatment (6a) DMEM containing 10% FCS (exponential growth) (6b) serum free (quiescent cells) (6c) TGF-β1 alone (5 ng/ml) (6d) Simvastatin (10 μM) alone (6e) Simvastatin (10 μM) and TGF-β1 (5 ng/ml) stimulation. FACS sorting was used to assess cell cycle progression using propidium iodide staining of cellular DNA content. Data are representative of FACS analysis performed in triplicate.