Cytokine/drug | Histamine response | Bradykinin response | NaF response |
---|
IL-1β | 97.0 (+/- 4.1) | 97.8 (+/- 4.3) | 85.9 (+/- 8.1) |
TNFα | 113.0 (+/- 8.4) | 127.4 (+/- 8.3) +** | 102.8 (+/- 11.9) |
IL-13 | 144.3 (+/- 9.3) +** | 113.8 (+/- 7.0) | 124.2 (+/- 15.6) |
IFNγ | 126.4 (+/- 7.5) ** | 128.0 (+/- 8.4) +** | 132.3 (+/- 14.0) |
Salmeterol | 117.7 (+/- 5.2) ** | 111.7 (+/- 5.0) * | 116.2 (+/- 10.9) |
Dexamethasone | 105.6 (+/- 4.4) | 100.3 (+/- 5.0) | 113.2 (+/- 10.4) |
- ASM cells were serum starved for 24 hours and then treated with medium alone, 10 ng/ml IL-1β, 10 ng/ml TNFα, 10 ng/ml IFNγ, 50 ng/ml IL-13, 1 μM dexamethasone or 1 μM salmeterol for 24 hours. Cells were loaded with [3H]-myo-inositol and IPx production following 100 μM Histamine, 0.1 μM bradykinin or 10 mM Sodium Fluoride stimulation was determined as described [30]. The IPx production in the medium alone treated cells was normalised to 100% and the other treatment groups determined relative to this. Data represents the mean response +/- S.E.M, n = 9 (three independent experiments for 3 donors). Data within groups was compared by ANOVA and post hoc Dunnett's Multiple Copmarison Test (+p < 0.05) or T-Test (*p < 0.05, **p < 0.01).