Figure 4From: NF-kappaB mediates the survival of human bronchial epithelial cells exposed to cigarette smoke extractSpecific suppression of p65 by siRNA. HBECs were transfected with control-siRNA, p65-siRNA or STAT3-siRNA for 6 hours as described in the methods. Cells were maintained in culture for additional 72 hours. Cell lysates were then subjected to immunoblot for p65, p50, STAT3 and β-actin. Data presented are one representative of 4 separate experiments.Back to article page