Figure 5
From: Increased levels of soluble interleukin-6 receptor and CCL3 in COPD sputum
Effect of CCL3 antagonism on migration of CD14+ monocytes towards COPD sputum supernatant. Assessment of CD14+ monocyte migration towards COPD PBS sputum supernatant in the presence of anti-human CCL3 mAb and anti-human CCR5 mAb. Peripheral blood CD14+ monocytes were isolated from three HNS. Chemotaxis is presented as the percentage of maximum chemotaxis (CD14+ monocytes added directly to wells of the lower insert of the chemotaxis plate) and is described as mean (SD). Data represents the results from three independently conducted experiments. Statistical significance of differences in %CD14+ monocyte chemotaxis observed between control (PBS sputum supernatant alone) and experimental conditions (+CCL3 mAb or + CCR5 mAb) was determined by application of paired parametric t-tests.