Figure 1

Expression of miR-708 in rh- TNF-α-treated ASM cells. A : Analysis of miRNAs that are predicted to target CD38 by q-PCR. Expression levels of these miRNAs in HASM cells with and without TNF-α treatment were normalized with small nuclear RNA U6. miRNAs with ct values above and below 34 in HASM cells are indicated by the horizontal line (n = 3–4 donors). B and C : miR-708 expression in HASM cells from non-asthmatics (NA-HASM) and asthmatics (AS-HASM), respectively. Rh-TNF-α caused a significant reduction in miR-708 expression in growth-arrested NA-HASM cells compared with cells treated with vehicle (n = 8 donors), while it caused a significant increase in its expression in AS-HASM cells (n = 6 donors). D : miR-708 expression in AS-HASM cells is higher than in NA-HASM cells both under vehicle and TNF-α-treated conditions (3–5 donors/group). Values are shown as mean ± SEM. C-vehicle-treated cells (0.1% BSA); T- rh-TNF-α (10 ng/ml) treated cells.