Figure 5

Smad2/3 nuclear translocation is Ca ²⁺dependent. (A) When HLMFs were incubated with Ca²⁺-free medium for 1 hour there was a significant reduction in the amount of Smad2/3 located in the nucleus, NFC n = 3 and IPF n = 3 (a minimum of 10 random cells were measured in one field for each donor, data pooled for NFC and IPF). (B) Representative immunofluorescent staining demonstrating the attenuated nuclear expression of Smad2/3 in Ca²⁺-free medium. Results are represented as mean ± SEM, *P < 0.05 (Paired t-test).