Skip to main content
Figure 3 | Respiratory Research

Figure 3

From: RETRACTED ARTICLE: Activation of c-Src tyrosine kinase mediated the degradation of occludin in ventilator-induced lung injury

Figure 3

Effect of occludin and c-Src expressions on 20% cyclic stretching with PP2 and DMSO. MLE-12 cells were exposed on 20% cyclic stretching for 4 h. A, B and C: Representative Western blotting of occludin and total and phosphorylated c-Src expressions and the density of proteins in non-stretching group was used as a standard to compare relative densities in the other groups. *P > 0.05, compared with the DMSO group. **P < 0.05, ##P < 0.05, #P < 0.05, compared with the PP2 group. Data are representative of 3 independent experiments. D: At the end of cyclic stretching, cells were fixed, blocked and then incubated with occludin primary antibody, performed overnight at 4°C. Cy3-Goat Anti-Rabbit IgG (Red) was used as the secondary antibody. Nuclei were counterstained with DAPI (Blue).

Back to article page