Figure 7
From: Astragalin inhibits autophagy-associated airway epithelial fibrosis
Inhibition of epithelial formation of autophagic vacuoles by astragalin. BEAS-2B cells were cultured with 20 μM H2O2 in the absence and presence of 1–20 μM astragalin for 4 h and incubated with monodansycadaverine (MDC) at 37°C for 10 min. MDC-loaded cells were visualized by fluorescence microscopy and the fluorescent intensity was quantified (A). The bar graphs (mean ± SEM, n = 3) represent quantitative results of the upper staining. Means without a common letter differ, P < 0.05. Immunofluorescence analysis showing inhibition of autophagosome formation by astragalin in OVA-challenged mouse lung tissues (B). Autophagosomes indentified as green staining was visualized with MDC and nuclear staining was done with 4′,6-diamidino-2-phenylindole. Each photograph is representative of four mice. Magnification: 200-fold.