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Fig. 1 | Respiratory Research

Fig. 1

From: The LIM-domain only protein 4 contributes to lung epithelial cell proliferation but is not essential for tumor progression

Fig. 1

Lmo4 is expressed in lung epithelial cells. a Immunofluorescence staining for Nkx2.1 (green) and Lmo4 (red) in embryonic lung. Scale bars = 25 μm. b Immunofluorescence staining for CC10, FoxJ1 and Lmo4 in adult lung. Scale bars = 50 μm. c Representative FACS plots showing the sorting strategy to isolate adult lung epithelial subpopulations from live CD31−CD45− cells. EpCAM−CD104−: non epithelial cells; EpCAM+CD104+ (also known as integrin β4): enriched in alveolar cells; EpCAM+CD104+CD24lo (CD24lo): enriched in club cells and progenitor cells; EpCAM+CD104+CD24hi (CD24hi): enriched in ciliated cells. d Representative photos of X-Gal and Nucleo-Fast Red counterstaining of sorted lung subpopulations isolated from Lmo4 LacZKI/+ mice. Scale bars = 50 μm

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Respiratory Research

ISSN: 1465-993X

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