Gene | Forward primer | Primer position | Reverse primer | Primer position | Product length [bp] | Annealing temp. [°C] |
---|---|---|---|---|---|---|
FUS1 | M: TGTTATCGTGGATTAGATATTGTTC | −559 to −482 | M: ACTATATTTTTACGATTACCACGCT | −375 to −349 | 207 | 56.5 |
U: TTATTGTGGATTAGATATTGTTTGT | −557 to −480 | U: ACTATATTTTTACAATTACCACACT | −375 to −349 | 205 | 56.5 | |
NPRL2/G21 | M: GTTCGGTTATTGTTATGGGTAGC | +108 to +131 | M: AACCAATTAAACTCTCGAAAACGT | +238 to +261 | 153 | 52.5 |
U: GGTTTGGTTATTGTTATGGGTAGTG | +107 to +132 | U: ACCAATTAAACTCTCAAAAACATC | +238 to +261 | 153 | 56.5 | |
RASSF1A | M: ATATTTTTTCGATTTGGAGTTTTTTC | −599 to −573 | M: CTACACTATAACCTACCCATCCTCG | −409 to – 384 | 215 | 56.5 |
U: TTTTTTTGATTTGGAGTTTTTTTGT | −599 to −573 | U: TACACTATAACCTACCCATCCTCAC | −410 to −385 | 211 | 57.5 |