Fig. 4

Neonatal infection with rA2-19F exacerbated Th2 and reduced effector CD8⁺ T cell responses in lungs. Pups were infected with media, rA2-19F or A2, and single cells were isolated from lungs and stained with CD3, CD4, CD8, IFNγ, and IL4 for flow cytometry at 6 dpi. a CD4⁺ T cells expressing IFNγ or IL4. N = 5-9. b CD4⁺ T cell subsets (Th1: IFNγ + IL4-; Th2: IFNγ-IL4+; multi-functional Th: IFNγ + IL4+). N = 5-9 (c) Representative flow plot of (a). d CD8⁺ T cell expressing IFNγ or IL4. N = 5-9. e Representative flow plot of (d). Sham: Neonates infected with media; A2: Neonates infected with A2 strain; rA2-19F: Neonates infected with rA2-19F. These figures are representative of 2 independent experiments. *:p < 0.05