Fig. 5

rA2-19F induced more pulmonary IL13 expression during primary infection. a–c Relative expression of IL4Rα on T cell subsets. Pups were infected with rA2-19F or A2, and single cells were isolated from lungs and stained with IL4Rα, CD3, CD4, IFNγ, and IL4 for flow cytometry at 6 dpi. MFI: mean fluorescence intensity. N = 7-9. d Relative expression of IL13 in the lungs. Pups were infected with rA2-19F, A2, or mock, and RNA from total lung homogenates was isolated at 5 h, 12 h, 2 days, and 6 days. The relative expression of IL13 in the infected lungs was determined using qPCR and normalized to corresponding mock infected lungs. N = 6-9. A2: Neonates infected with A2 strain; rA2-19F: Neonates infected with rA2-19F. These figures are representative of 2 independent experiments. *:p < 0.05