Fig. 1
Western blot analysis of CADM1 expression in IIP lungs. a Protein was extracted from control and IIP lung sections and subjected to Western blot analyses using an anti-CADM1 antibody. Cases are numbered as in Additional file 1: Table S1. Results of the remaining cases are shown in Additional file 2: Figure S1. Arrowheads indicate bands corresponding to the full-length, αCTF, and βCTF forms of CADM1. The blot was reprobed with an anti-CK7 and anti-TTF1 antibody to estimate the number of epithelial cells and AECIIs, respectively. An asterisk indicates the sample that did not gave a clear immunoreactive band for TTF1. After Western blot transfer, the gels were stained with CBB to indicate the amount of protein loading per lane. b CADM1 ectodomain shedding rates (amount of CTFs relative to full-length CADM1) and c full-length CADM1 level per lung epithelial cell (relative to CK7) in each patient sample are plotted as dots. Statistical significance between two groups was analyzed using the Steel–Dwass test. P-values ≤ 0.05 are shown. d The full-length CADM1 level per epithelial cell with the CADM1α-shedding rate is shown in a scatter plot, and the dot distribution approximates a linear function (dotted line). Correlations and statistical significance were analyzed using Spearman’s rank test. R 2 and P-values are shown