Fig. 2

Localization of phospho-Akt and -Stat3 and expression of IL-6 at early inflammatory stage of BLM-injured lung. a Lung sections of mice instilled with PBS or BLM (3 dpi) were stained with anti-phospho-Akt or anti-phospho-Stat3 antibody in combination with anti-proSP-C antibody followed by reaction with Alexa 488- or Alexa 594-conjugated second antibodies (488 for phospho-Akt and -Stat3; 594 for SP-C). Arrowheads indicate phospho-Akt⁺SP-C⁺ and phospho-Stat3⁺SP-C⁺ cells. b Lung sections from mice instilled with BLM (3 dpi) were stained with anti-IL-6 antibody in combination with anti-proSP-C or anti-Iba1 antibody followed by reaction with Alexa 488- or Alexa 594-conjugated second antibodies (488 for IL-6; 594 for SP-C and Iba1). Arrowheads indicate IL-6⁺SP-C⁺ and IL-6⁺Iba1⁺ cells. Asterisk indicates a cell with macrophage-like morphological features. c Lung sections from mice instilled with BLM (3 dpi) were stained with anti-IL-6, anti-proSP-C and anti-Iba1 antibodies followed by reaction with Alexa 350-, 488- and Alexa 594-conjugated second antibodies. Data are shown as mean ± S.E.M. (n = 6). Nuclei were stained with DAPI. Similar results to the immunofluorescence profiles in (a) and (b) were observed in four independent experiments