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Fig. 2 | Respiratory Research

Fig. 2

From: A HuR/TGF-β1 feedback circuit regulates airway remodeling in airway smooth muscle cells

Fig. 2

PDGF treatment promoted TGF-β1 along with Col-I and α-SMA expression. ad HuR, TGF-β1, Col-I and α-SMA mRNA levels were measured by RT-PCR and protein levels were measured by western blotting in ASM cells under the stimulation of PDGF for various durations (0, 6, 12, 24 h). GAPDH in RT-PCR and β-actin in western blotting were the internal control. Density ratio of bands in western blotting was calculated and represented as relative expression level. Each value represents the mean ± SD of three independent experiments. e Immunofluorescence analysis was used to detect subcellular distribution of HuR and TGF-β1 in ASM cells following the same treatment. *P < 0.05 compared with control; **P < 0.01 compared with control

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