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Fig. 3 | Respiratory Research

Fig. 3

From: A HuR/TGF-β1 feedback circuit regulates airway remodeling in airway smooth muscle cells

Fig. 3

HuR silencing changed the function of ASM cells. a FACS analysis of the apoptotic and necrotic cells using Annexin V/PI after cultured for 0 h or 12 h in the Consi and HuRsi groups. Annexin V/PI staining with FACScan dot plot analysis were used to divide the treated and control cells into three groups: living cells (lower left quadrant), necrotic cells (upper right quadrant). b Knockdown efficiency of HuR was analyzed by RT-PCR and western blot assay. GAPDH in RT-PCR and β-actin in western blotting were the internal control. Density ratio of bands in western blotting was calculated and represented as relative expression level. *P < 0.05 compared with control; #P < 0.05 control siRNA vs. HuR siRNA upon the 12 h stimulation of PDGF

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