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Fig. 4 | Respiratory Research

Fig. 4

From: A HuR/TGF-β1 feedback circuit regulates airway remodeling in airway smooth muscle cells

Fig. 4

HuR silencing attenuated TGF-β1 along with Col-I and α-SMA expression and decreased the mRNA stability of TGF-β1. a Silenced HuR downregulated endogenous TGF-β1 protein expression at a time-dependent manner. Density of each band was quantified using Image software and the ratio of bands was calculated and represented as relative expression level. b The concentration of TGF-β1 in the cultured serum was detected by ELISA kits. Data was analysed by Curve Expert and showed by mean ± SD. c Remaining TGF-β1 mRNA were detected in siHuR or Consi group with stimulation of actinomycinD for 0, 4, 8,12 h. RNA expression was 50 % of the initial level [t1/2 = Ln(0.5)/slope]) representing the half-life. Graphpad prism5.0 was used to analyse data and data was shown with mean ± SD. d Western blotting showed significant decrease of Col-I after the knockdown of HuR. The outcomes of the concentration of Col-I in the cultured serum was in consist with western blotting. e Western blotting demonstrated alleviatin of α-SMA expression after silencing HuR. *P < 0.05 compared with control; #P < 0.05 control siRNA vs. HuR siRNA upon the stimulation of PDGF for 12 h or 24 h

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