Fig. 4

LPS-induced mucous cell metaplasia is augmented in LPS-pretreated athymic mice compared to euthymic mice. a Representative photomicrographs of axial airway sections stained with AB/H&E from S/0, S/100, L0 and L/100 Foxn1^WT mice compared with respective Foxn1^nu mice. Quantification of the number of mucous cells (b) and the number of airway epithelial cells (c) analyzed per mm BL. d Representative micrographs of axial airway sections showing Scgb1a1 (green) and Muc5ac (red) positivity with DAPI-stained nuclei (blue) in S/100 and L/100 Foxn1^WT and Foxn1^nu mice. e Quantification of the number of Scgb1a1- and Muc5ac-positive cells in S/100 and L/100 Foxn1^WT and Foxn1^nu mice. f Mean florescence intensity (MFI) of Scgb1a1- and Muc5ac-positive cells in S/100 and L/100 Foxn1^WT and Foxn1^nu mice. Error bar indicates mean ± SEM (n = 6 per group). * P < 0.05, ** P < 0.01 compared to S/0 unless otherwise indicated