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Fig. 9 | Respiratory Research

Fig. 9

From: Proteases and oxidant stress control organic dust induction of inflammatory gene expression in lung epithelial cells

Fig. 9

Effects of protease inhibitor and antioxidants on the activation of NFκB-p65. a Beas2B cells were treated with medium (C), 1 % dust extract (DE), 25 μg/ml soybean trypsin inhibitor (SBTI), or a combination of DE and SBTI for 5 min. A representative Western blot depicting the levels of phosphorylated NFκB-p65 and total NFκB-p65 is shown. c and e Beas2B cells were incubated first with medium (C), 0.5 μM CDDOIm or 15 mM n-acetylcysteine (NAC) adjusted to pH 7.0 for 3 h or 30 mM dimethylthiourea (DMTU) for 1 h and then treated with or without 1 % dust extract for 5 min. b, d and f The levels of phosphorylated NFκB-p65 were normalized to total NFκB-p65 and the levels in cells treated with medium (C) were arbitrarily considered as 100 and relative levels in treated cells are shown. Data shown are means ± SE (n = 3). * P < 0.05

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