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Fig. 1 | Respiratory Research

Fig. 1

From: Differences in systemic adaptive immunity contribute to the ‘frequent exacerbator’ COPD phenotype

Fig. 1

Gating strategy for adaptive immune cells. Lymphocytes were gated based on their characteristic scatter patterns. Lymphocytes were then classified based on CD3 expression to identify T cells, which were divided into CD4+ and CD8+ T cells, prior to separation into their four main subsets of naive, central memory (Tcm), effector memory (Tem), and end-stage T cells (Tend); this separation was based on the expression of CD45R0 and CD62L. Tcm cells: CD45R0+/CD62L+, Tem cells: CD45R0+/CD62-, Naive T cells: CD45R0-/CD62L+, and Tend cells: CD45R0-/CD62L-. The CD4+ and CD8+ T cells were subsequently analysed (including each of the main subsets) for expression of the markers CD127, HLA-DR, CD25 and PD-1. Regulatory T-cells were identified based on the high expression of CD25 and low expression of CD127 in CD4+ T cells

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