Fig. 2
Representative immunoblot analyses using antibodies against the dopamine D1 receptor using total protein prepared from freshly dissected native human tracheal epithelium (20 μg), primary cultured human airway epithelial cells (100 μg), the human bronchial epithelial cell line (16HBE14o-) (100 μg), the human pulmonary mucoepidermoid carcinoma cell line (NCI-H292) (100 μg), and human airway smooth muscle cells (positive control) (100 μg). Reprobing of blots for GAPDH was performed to demonstrate relative lane loading. Each image is representative of at least 3 independent immunoblots