Fig. 6

Chronic electron transport chain inhibition induced a glycolytic shift in lungs. Our data indicate accumulation of glucose (a) and glyceraldehyde 3-phosphate (b) at day 12 of study. In contrast, 3-phosphoglycerate (c) and final product of glycolysis – lactate (d) – possess a progressive increase that becomes significant by day 24 for 3-phosphoglycerate and both day 12 and 24 for lactate. However, an upstream pentose phosphate intermediate – ribulose-5-phosphate (e) has gradually decreased with a disease progression with a significant reduction by day 24. Finally, the content of ribose (f) in the lungs was also strongly elevated at day 12 and 24, which may represent the ribose to glyceraldehyde-3-phosphate shuttle that contributes to upregulation of pulmonary glycolysis. Data are mean ± SEM, N = 4–7 per group. *P < 0.05 vs. Control in 1-way ANOVA