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Table 1 Demographics of the study population and biologic samplesa

From: Altered lung biology of healthy never smokers following acute inhalation of E-cigarettes

Parameter

Total cohort of n = 10

Sub-cohort exposed to EC with nicotine, n = 7

Sub-cohort exposed to EC without nicotine, n = 3

Baselineb

Post-ECc

Baselineb

Post-ECc

Baselineb

Post-ECc

n

10

7

3

Sex (male/female)

5/5

4/3

1/2

Age (yr)

40.2 ± 9.7

40.4 ± 11.2

39.7 ± 6.7

Race (B/W/H/O)d

7/0/3/0

6/0/1/0

1/0/2/0

Smoking history

None

None

None

Pulmonary functione

 FVC (% predicted)

110 ± 14

107 ± 13

112 ± 16

112 ± 11

105 ± 6

98.3 ± 12

 FEV1 (% predicted)

110 ± 14

107 ± 15

112 ± 15

113 ± 11

103 ± 9

91 ± 8

 FEV1/FVC (% observed)

81 ± 33

81 ± 4

81 ± 3

83 ± 3

81 ± 4

76 ± 4

 TLC (% predicted)

92 ± 11

92 ± 11

91 ± 11

92 ± 7

94 ± 13

91 ± 21

 DLCO (% predicted)

89 ± 10

86 ± 10

88 ± 10

85 ± 13

92 ± 9

87 ± 3

 O2 saturation

99 ± 1

99 ± 1

99 ± 1

99 ± 1

99 ± 2

98 ± 1

Bronchoalveolar lavage

 % recovery

61.7%

64.8%

64.4%

65.5%

56.2%

62.9%

 Total cells recovered (x106)f

12.1 ± 7.9

10.0 ± 6.7

10.6 ± 5.3

11.0 ± 7.6

15.5 ± 13.1

6.6 ± 2.5

 Epithelial cells (%)g

87.0 ± 9.0

1.6 ± 1.7

1.1 ± 1.0

2.0 ± 1.8

0.3 ± 0.3

0.6 ± 0.5

 Macrophages (%)g

92.7 ± 7.4

90.0 ± 5.7

91.1 ± 8.3

90.0 ± 6.2

96.6 ± 2.7

90.1 ± 5.6

 Lymphocytes (%)g

5.8 ± 6.5

7.8 ± 4.8

7.2 ± 7.4

7.5 ± 4.7

2.6 ± 2.1

8.5 ± 6.2

 Neutrophils (%)g

0.5 ± 0.5

0.50 ± 0.3

0.6 ± 0.5

0.5 ± 0.2

0.3 ± 0.4

0.5 ± 0.7

 Eosinophils (%)g

0.1 ± 0.2

0.2 ± 0.2

0.1 ± 0.2

0.1 ± 0.2

0.1 ± 0.2

0.3 ± 0.1

Small airway epitheliumh

 Epithelial cells (%)

99.1 ± 1.0

99.3 ± 1.0

99.2 ± 1.0

99.4 ± 0.5

98.9 ± 1.2

99.0 ± 1.8

 Inflammatory cells (%)

0.86 ± 1.0

0.7 ± 1.0

0.8 ± 1.0

0.6 ± 0.5

1.1 ± 1.2

1.0 ± 1.8

 Ciliated cells (%)

60.1 ± 4.5

58.3 ± 7.5

58.6 ± 4.1

58.3 ± 6.8

63.7 ± 3.4

58.2 ± 10.9

 Secretory cells (%)

10.5 ± 3.4

11.1 ± 2.3

9.2 ± 2.4

10.7 ± 2.0

13.4 ± 4.1

12.1 ± 3.0

 Undifferentiated cells (%)

22.3 ± 4.3

21.9 ± 6.6

22.6 ± 4.7

20.2 ± 4.3

23.3 ± 3.3

26.0 ± 10.1

 Basal cells (%)

6.92 ± 3.7

7.9 ± 5.9

8.7 ± 2.7

10.3 ± 5.5

2.8 ± 2.2

2.5 ± 1.3

  1. aAll subjects were healthy never smokers; values shown are mean ± standard deviation unless otherwise noted
  2. bBaseline – assessment at baseline prior to EC exposure
  3. cPost-EC – assessment 1 week after baseline, following 2 × 10 puffs of Blu brand electronic cigarette with (n = 7 subjects) or without (n = 3 subjects) nicotine
  4. dEthnicity is indicated as: Black (B), White (W), Hispanic (H), Other (O)
  5. eLung function values shown are pre-bronchodilator. FVC - forced vital capacity; FEV1 - forced expiratory volume in 1 s; DLCO - diffusion capacity for carbon monoxide; TLC - total lung capacity; all values are presented as % predicted except for FEV1/FVC presented as % observed
  6. f% recovery – volume recovered / volume infused
  7. gDifferential cell count prior to enrichment of alveolar macrophages (see Methods for details); after enrichment, alveolar macrophages represent ≥98% of the cell population used for RNA sequencing
  8. hCell differentials of the small airway epithelium used for RNA sequencing