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Fig. 4 | Respiratory Research

Fig. 4

From: RETRACTED ARTICLE: The anti-proliferative and anti-inflammatory response of COPD airway smooth muscle cells to hydrogen sulfide

Fig. 4

Effect of inhibiting cystathionine-β-synthase (CBS) on ASM from non-smokers, smokers and COPD patients. ASM cells were incubated with 1 mM of an inhibitor of CBS (O-(carboxymethyl)-hydroxylamine hemihydrochloride [CHH]) for 30 min; media with 2.5% FCS was added for a further 8 days with NaSH (100 μM). DNA synthesis (a), IL-6 release (b), and CXCL8 release (c) were subsequently measured by bromodeoxyuridine (BrdU) ELISA and DuoSet ELISA respectively. Bars represent means (± SEM) of nine ASM donors. */+/#P < 0.05; **/++/##P < 0.01; ***/+++/###P < 0.001

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