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Fig. 2 | Respiratory Research

Fig. 2

From: Unfractionated heparin ameliorates pulmonary microvascular endothelial barrier dysfunction via microtubule stabilization in acute lung injury

Fig. 2

Effect of LPS on cell viability and permeability. a Cell viability was measured using the cell viability assay after stimulation with different concentrations LPS (0.1 μg/ml, 1 μg /ml, 5 μg/ml, 10 μg/ml, 20 μg/ml, 50 μg/ml, or 100 μg/ml) for 24 h. The viability of HPMECs was slightly reduced by 10 μg/ml LPS and significantly decreased by ≥20 μg/ml LPS. b Cell permeability was measured by the TEER across HPMECs after LPS (10 μg/ml) stimulation for different time periods (1, 3, 6, 9, 12, or 24 h). HPMECs stimulated by LPS for more than 6 h showed marked decrease in the TEER of HPMEC monolayer. Values are means ± SD of five independent experiments. t-test, * P < 0.05 compared to the vehicle group. t-test, ** P < 0.01 compared to the vehicle group. t-test, *** P < 0.001 compared to the vehicle group

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