Fig. 4
From: Mitochondrial creatine kinase 1 in non-small cell lung cancer progression and hypoxia adaptation
Hypoxia induces the expression of CKMT1 in NSCLC cells. A The protein levels of HIF-1α and CKMT1 in H1650 and H1299 cells exposed to hypoxia for 6, 12, 24 and 48 h by western blot. B The wild (CKMT1-promoter-WT) or mutated (CKMT1-promoter-MUT) binding sequence of the CKMT1 promoter region to transcription factor HIF-1 was predicted with the PROMO 3.0 website. C The relative luciferase activety in normoxia or hypoxia. D The protein levels of HIF-1α and CKMT1 in H1650 and H1299 cells exposed to hypoxia and LW6 (HIF-1α inhibitor) treatment were detected by western blot. *P < 0.05, compared to NC; #P < 0.05, compared to Hypoxia