Fig. 4

Eosinophils and group 2 innate lymphoid cells are present at increased numbers in pre-existing allergic airway inflammation that is amplified by RSV infection. Eosinophils, neutrophils, group 2 innate lymphoid cells (ILC2) and group 3 innate lymphoid cells were quantitated in BALF at day 24 and 26 by flow cytometry. A–C, E Eosinophils and A–C, G neutrophils were identified as SSC + CD11c-CD11b + Siglec-F + and SSC + CD11c-CD11b + Ly6C + cells, respectively. D ILC2 and F ILC3 were identified as in Fig. 3. Cell counts were determined using the % total cells acquired in relative gates and multiplied by the total number of cells acquired from each lavage. Data represent five separate experiments (N = 5–6 mice/group for day 2, and N = 10–12 mice/group for day 4). Significance * indicates P < 0.05 between treatment groups